Research Interestspelvic organ prolapse, pre-term birth
Major research focus:
I. The regulation of myometrial functions during pregnancy and labour, specifically the role of mechanical and endocrine signals. My research goal is to investigate (A) the mechanisms of myometrial activation during the initiation of preterm and term labour. (B) The role of leukocyte-mediated myometrial inflammation in the initiation of labour. These investigations include the use of human and animal tissues, primary uterine smooth muscle cells, myometrial and endothelial cell lines. Cultured myocytes are used to investigate cellular and molecular responses. Whole animals are used to verify that the mechanisms, identified with the in vitro systems, are effective in vivo.
II. The study of molecular mechanisms underlying the development of Pelvic Organ Prolapse (POP) in pre- and post-menopausal women – in collaboration with Drs May Alarab and Harold Drutz, Division of Urogynecology and Reconstructive Pelvic Surgery in Mount Sinai Hospital, Toronto. Our goal is to characterise (1) the contribution of mechanical stretch on the development of Pelvic Organ Prolapse and (2) the potential protective effects of ovarian hormones on vaginal tissue deterioration. This knowledge will expand the diagnostic and clinical treatment capability; will provide strategies identifying women at risk of developing POP and will determine whether risk factor modification prevents the development of pelvic floor disorders in such women improving their life quality and reducing general health care costs.
2014 – 2018 ~ Burroughs Wellcome Fund Preterm Birth Initiative. “Targeting leukocyte activation to prevent preterm labor”. $150,000 per annum / Total: $600,000 (1/7/14-31/6/18). Drs. S.J. Lye (PI), K. Adams, M. Gravett and O. Shynlova.
2014 – 2017 ~ March of Dimes Birth Defects Foundation. “Prevention of Preterm Birth: Targeting Inflammatory Pathways”. $100,000 per annum / Total: $300,000 (1/3/14-31/2/17). Drs. S.J. Lye (PI), A. Bocking, S. Kim and O. Shynlova.
2010 – 2015 ~ Canadian Institutes of Health Research (MOP-37775). “Control of Myometrial Contractile Activity during Pregnancy”. $182,464 per annum / Total: $912,320 (1/9/10-31/8/15). Drs. S.J. Lye (PI), O. Shynlova.
2012 – 2015 ~ Research Fund, Department of Obstetrics and Gynecology at Mount Sinai Hospital, University Health Network “Vaginal Stretch and Ovarian Hormones: Potential Implications for the Development of Pelvic Organ Prolapse”. $25,000 per annum / Total: $50,000 (07/12 – 09/15). Drs. O. Shynlova (PI), M. Alarab.
Publications and Awards
Medel S., Alarab M., Kufaishi H., Drutz H., Shynlova O. (2015) Attachment of primary vaginal fibroblasts to absorbable and non-absorbable implant materials coated with Platelet Rich Plasma: potential application in Pelvic Organ Prolapse surgery. Female Pelvic Medicine & Reconstructive Surgery (accepted , January 2015)
Srikhajon K.*, Shynlova O.*, Preechapornprasert A., Chanrachakul B., Lye S. A (2014) New Role for Monocytes in Modulating Myometrial Inflammation During Human Labor. Biol Reprod. Jul;91(1):10 (*co-first authors).
Shynlova O., Dorogin A., Li Y., Lye S. (2014) Broad Spectrum Chemokine Inhibitor Delays Infection-induced Preterm Delivery in Mice. J Cell Mol Med. Sep;18(9):1816-29
Lee Yu-H, Shynlova O, Lye S. (2014) Stretch-induced human myometrial cytokines enhance immune cell recruitment via endothelial activation - the model of physiologic myometrial inflammation, Cel Mol Immunology. Jun 2. doi: 10.1038/cmi.2014.39. [Epub ahead of print]
Alarab M, H.Kufaishi, Drutz H, Lye S, Shynlova O. (2014) Extracellular matrix remodeling in vaginal tissue of premenopausal women with pelvic organ prolapse. Reproductive Sciences, Jun; 21(6):704-15.
Shynlova O, Bortolini MA, Alarab M. (2013) Genes responsible for vaginal extracelluar matrix metabolism are modulated by women’s reproductive cycle and menopause, Int Braz Journal of Urology, Mar-Apr; 39(2):257-67.
Shynlova O, T. Nedd-Roderique, Y. Li, A. Dorogin, Nguyen T. and S. J. Lye. (2013) Infiltration of myeloid cells into decidua is a critical early event in the labour cascade and post-partum uterine remodeling, Journal of Cellular& Molecular Medicine, J Cell Mol Med. Feb;17(2): 311-24.
Shynlova O., T. Nedd-Roderique, Y. Li, A. Dorogin and S. J. Lye. (2013) Myometrial immune cells contribute to term parturition, inflammation-associated preterm labour and post-partum involution in mice (Journal of Cellular& Molecular Medicine, Jan;17(1):90-102.
Shynlova O. and S. J. Lye (2013) Regulation of Parturition in “The Guide for Investigations of Mouse Pregnancy” Academic Press/Elsevier. Chapter 1: pp 2-13.
Shynlova O., Yu-H. Lee, K. Srikhajon and S. J. Lye. (2013) Physiologic Uterine Myometrial Inflammation and Labor Onset: Integration of Endocrine and Mechanical Signals. Reproductive Sciences, Feb;20(2):154-67
Xie N, Li Y, C Yu, Lam S, Liu L, Shynlova O, Challis J, Lye S, X. Dong (2012) Expression and Function of Myometrial PSF Suggest a Role in Progesterone Withdrawal and the Initiation of Labour. Molecular Endocrinology; 26(8):1370-9.
Alarab M, Bortolini MA, Drutz H, Lye S, Shynlova O. (2010) LOX family enzymes expression in vaginal tissue of premenopausal women with severe pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct. Nov; 21(11):1397-404.
Shynlova O., A. Dorogin, S. J. Lye (2010) Stretch-Induced Uterine Myocyte Differentiation during Rat Pregnancy: Involvement of Caspase Activation. Biology of Reproduction, Jun;82(6):1248-55
Shynlova O., R. Kwong, S. J. Lye. (2010) Mechanical Stretch Regulates Hypertrophic Phenotype of the Myometrium during Pregnancy, Reproduction, 39(1):247-253
Shynlova O, Tsui, S. Jaffer, S. J. Lye (2009) Integration of endocrine and mechanical signals in the regulation of myometrial functions during pregnancy and labour. European Journal of Obstetrics, Gynecology & Reproductive Biology, May;144 Suppl 1:S2-10